RNA tectonics: towards RNA design

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Enhancement of RNA Interference Effect in P19 EC Cells by an RNA-dependent RNA Polymerase

Background: RNA interference (RNAi) is a phenomenon uses double-stranded RNA (dsRNA) to specifically inhibit gene expression. The non-specific silencing caused by interferon response to dsRNA in mammalian cells limits the potential of utilizing RNAi to study gene function. Duplexes of 21-nucleotide short interfering dsRNA (siRNA) inhibit gene expression by RNAi. In some organisms, siRNA can als...

RNA-LEGO: Combinatorial Design of Pseudoknot RNA

In this paper we enumerate k-noncrossing RNA pseudoknot structures with given minimum stack-length. We show that the numbers of k-noncrossing structures without isolated base pairs are significantly smaller than the number of all k-noncrossing structures. In particular we prove that the number of 3and 4-noncrossing RNA structures with stack-length ≥ 2 is for large n given by 311.2470 4! n(n−1)....

RNA nanotechnology breakthrough for targeted release of RNA-based drugs using cell-based aptamers

Nucleic acids play different roles besides storing information and proteins coding. For example, single-stranded nucleic acids can fold into complicated structures with capability of molecular detection, catalyzing bioreactions and therapy. The development of RNA-based therapies has been rapidly progressed in the recent years. RNA aptamers are biomolecules with a size of 10 to 50 nm that can be...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*